Selective Binding of Organophosphate Pesticides Using Molecular Imprinted Polymers

Molecular Imprinted Polymers (MIPs) have been used for recognition and binding of different compounds. We are developing MIPs to selectively bind organophosphate pesticides and toxic chemical warfare nerve agents. MIPs were made to the pesticide Dichlorvos. Control MIPs were made without the template. Dichlorvos-MIPs bound more Dichlorvos in comparison to control-MIPs. The Dichlorvos-MIPs can be regenerated after binding the pesticide and reused at least three times by repeating the washing procedure. Dichlorvos-MIPs demonstrated specificity for its template since these MIPs did not bind Methamidophos, Phosdrin, and Metasystox I, compounds with similar chemical structures to that of Dichlorvos. INTRODUCTION Molecular Imprinted Polymers (MIPs) are highly cross-linked polymers, which are formed by crosslinking monomer in the presence of a template. The template molecule interacts with the monomer (usually methacrylic acid for a basic template molecule and divinyl pyridine for an acidic template molecule) in solution either covalently or non-covalently and highly cross-linked polymers form around the template. For example, a template molecule is dissolved in a progen (solvent), the template selfassembles with the monomer when mixed with cross-linker and polymerizes with the addition of an initiator. Following removal of the template molecule, the resultant polymers possess the steric and chemical memory for the recognition of that template (Figure 1). Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. 1. REPORT DATE 01 OCT 2005 2. REPORT TYPE N/A 3. DATES COVERED 4. TITLE AND SUBTITLE Selective Binding Of Organophosphate Pesticides Using Molecular Imprinted Polymers 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Division of Biochemistry, Department of Biochemical Pharmacology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910-7500 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 12. DISTRIBUTION/AVAILABILITY STATEMENT Approved for public release, distribution unlimited 13. SUPPLEMENTARY NOTES See also ADM001851, Proceedings of the 2003 Joint Service Scientific Conference on Chemical & Biological Defense Research, 17-20 November 2003. 14. ABSTRACT 15. SUBJECT TERMS 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT UU 18. NUMBER OF PAGES 6 19a. NAME OF RESPONSIBLE PERSON a. REPORT unclassified b. ABSTRACT unclassified c. THIS PAGE unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18